Radhika, V

Designation: 
Scientist (SS)
Division/Section: 
Division of Biotechnology
Specialisation: 
Computer Application in Agriculture
Experience (No. of Years): 
13
Permanent Address: 
Same as present address
Research Accomplishments: 

* In silico mining for molecular markers in horticultural crops * EST-SSR, SNP database of onion http://iihr.ernet.in/onionest/ *Software for disease prediction in horticultural crops * e-module on Carnation cultivation. http://www.iihr.ernet.in/carnation/ * Software for obtaining pedigree and other information in livestock * Software for digestibility trial calculations in livestock * Development of databases, web-based information systems, e-modules

Important Publications: 

*Radhika V et al. (2013) In silico identification and validation of microsatellite markers from onion EST sequences, Journal of Horticultural Science & Biotechnology, 88(5):664–670 . http://www.jhortscib.org/Vol88/88_5/23.htm

* Radhika V et al. (2011) In silico microsatellite development in arum lily, Journal of Horticultural Sciences, 6(1): 37-40. http://www.iihr.ernet.in:8081/A2gb/JHS_6(1)_2011.pdf

* Radhika V et al. (2011) In Silico mining of single nucleotide polymorphisms in expressed sequence tags dataset of Allium cepa L., Online Journal of Bioinformatics, 12 (1): 34-45. http://users.comcen.com.au/~journals/aliumcepaabs2011.htm

* D. C. Lakshmana Reddy, V. Radhika, A. Bharadwaj, K. S. Khandagale and C. Aswath (2012) Mirnas in brinjal (solanum melongena) mined through an in silico approach. Journal of Horticultural Science & Biotechnology, 87 (2) 186-192. http://www.jhortscib.org/Vol87/87_2/16.htm

* Kanupriya, Shobhana M, Aswath C, Radhika V, Laxman Reddy and Vasugi C. (2012) Genetic Relationship among Thirteen Cultivars of Papaya (Carica papaya L.) and two species of Vasconcellea (Wild Papaya) using Molecular Markers, Indian Journal of Agricultural Sciences. http://epubs.icar.org.in/ejournal/index.php/IJAgS/issue/view/536

* Rao S B N, Radhika V, Singh N and Dutta T K (2011) Evaluation of mineral adequacy of natural browse species and concentrate ingredients for goats. Livestock Research for Rural Development. Volume 23, Article #166. http://www.lrrd.org/lrrd23/8/rao23166.htm

* V.Radhika, S.B.N.Rao (2010) Formulation of Low Cost Balanced Ration for Livestock using Microsoft Excel, Wayamba Journal of Animal Science Number: 1281076233 http://www.wayambajournal.com/papers/1281076233.php

* V.Radhika, M.R.Hegde (2010) Web-Enabled Information System for Research Management and Communication, Journal of Communication studies 28(2): 31-35

* V.Radhika, Nita Khandekar, M.R.Hegde (2009) Application of E-learning in Horticulture, Journal of Communication Studies 28(3): 43-46

* V.Radhika and R.Roy (2006) Software for obtaining pedigree and related information, Indian Journal for Animal Sciences 76(3): 273-275 http://agris.fao.org/agris-search/search/display.do?f=2008/IN/IN0801.xml...

* V.Radhika, S.K.Singh and R.Roy (2006) A Database system for efficient data management in a livestock breeding farm, Indian Journal of Dairy and Biosciences, 15(1 & 2): 85 – 91

* S.B.Nageswara Rao, R.C.Chopra and V.Radhika (2004) Sodium Bentonite or activated charcoal supplementation on dry matter intake and growth rate of young goats fed diets with aflatoxin B 1, Indian Journal of animal Sciences, 74(3): 324-326, March 2004

* S.B.Nageswara Rao, R.C.Chopra and V.Radhika (2004) Effect of supplementing Bentonite or Activated charcoal on certain blood Parameters of young goats fed diets with or without added Aflatoxin B1 , Animal Nutrition and Feed Technology 2004(4) : 83-90, April 2004

* V.Radhika, S.B.N.Rao, R.Roy (2003) A Software Program for Digestibility trial Calculations, Indian Journal of Animal Sciences 73(11): 1285-1288

.....

Papers presented in Seminars/ Symposia etc.
* Radhika V, Reddy DCL, LL Lobo and C Aswath (2013) Analysis and characterization of microsatellites mined in-silico in Allium cepa. Compendium of Accelerating Biology 2013: The Next Wave, Centre for Development of Advanced Computing, Pune, 20-22 February 2013, pp 105-106.
* Radhika V and C Aswath (2012) In silico mining of genes associated with disease resistance in horticultural crops. Proceedings of National Workshop on Bioinformatics Techniques in Drug Designing, St. Joseph’s Post Graduate and Research Centre, Bangalore, 19-20 January 2012, pp 11-14.
* Shweta, Radhika V, Archana, DC Lakshmana Reddy and Aswath C (2010). In silico mining of microsatellites from expressed sequence tags of Arum lily. National Consultative Meet on Bioinformatics in Horticulture held at Indian Institute of Spices Research, Calicut during 9-10 October 2010, pp 115.
* Archana, Lakshmana Reddy DC, Shweta, Radhika V and Aswath C (2010). In silico mining and distribution studies of ESTSSRs in Myrtaceae family from EST dataset. Proceedings of National Consultative Meet on Bioinformatics in Horticulture held at Indian Institute of Spices Research, Calicut, 9-10 October 2010, pp 105.

Awards & Recognitions, Patents, Inventions, etc: 

* NBHM scholarship in MSc * Awarded various prizes including M.S.Huzurbazar Memorial Prize and S.I.E.S. President’s Gold Medal for scoring highest marks in BSc

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Contact Details
Address for Communication: 
Division of Biotechnology, Indian institute of Horticultural Research, Hessaraghatta Lake PO, Bangalore - 560089
Email Address: 
vr@iihr.ernet.in
Telephone #: 
+91-80-28466420

Lakshmana Reddy, D.C.

Designation: 
Scientist( Agril. Biotechnology)
Division/Section: 
Division of Biotechnology
Specialisation: 
Molecular Biology
Qualification: 
Ph.D
Experience (No. of Years): 
06
Research Accomplishments: 

Brief summary of current areas of research: Presently am working on the three following aspects of molecular markers and the mining of molecular markers in next generation sequencing data. 1.Development of SCAR Markers: Here mainly focus on development of SCAR markers either from RAPDs or from SRAPs polymorphism. Presently 23 SCAR markers are developed in Ridge gourd related to Downey mildew resistance, 11 SCAR markers are developed in eggplant related to Ralostonia wilt resistance,6 SCAR markers are developed in citrus rootstocks validation and validated successfully and patent filing under process. 2.Validation of Available trait linked functional markers: Here, public available trait linked functional molecular markers used in main stream crop breeding programme. we are successful in validation of powdery mildew resistance linked SCAR /SSR markers in pea, mitochondrial gene specific primers linked to male sterility trait in carrot, Fusarium linked CAPs markers in watermelon and rust resistance molecular markers in French bean. 3. Construction of linkage maps Here , main focus on development of linkage maps to Ralstonia resistance wilt in eggplant, Fusarium wilt resistance in watermelon and mining of EST-SSR , Genomic SSR, SNPs from sequencing data set in most of the horticultural crops by using various bioinformatic tools

Important Publications: 

Lakshmana Reddy D C, Archana Bhardwaj, Radhika V and Aswath C 2012, “miRNAs in Solanum melongena–mined through In silico approach” Journal of Horticulture Science and Biotechnology, UK, Vol 87(2): 186-192 Lakshmana Reddy D C Srikanth H S, Aswath C and Singh T.H., 2012. SSR based DNA finger printing bar codes for eggplant varieties and hybrids. International Journal of vegetable science, 18:1-12 Taylor and Francis, UK D.C.Lakshmana Reddy, H .M. Mahadeva Swamy, R. Asokan, S.C.Venkatesh and T.K.S Gowda 2012., Isolation, Molecular characterization and diversity of Lepidopteron active cry type genes of Baillus thuringiensis isolates and their efficacy against Helicoverpa armigera Hubner( Noctuidae: Lepidoptera), Biopesticide International, Vol-8(2):93-106. Radhika V, Reddy DCL, Bhardwaj A, Aswath C 2011 In Silico mining of single nucleotide polymorphisms in expressed sequence tags dataset of Allium cepa L.., Online Journal of Bioinformatics . Volume 12(1):34-45, Radika V, Lakshmana Reddy D C, Archana B, Shewta and Aswath C., 2011, In silico microsatellite development in Arum lily (Zantedeschia aethiopica), Journal of Horticultural Sciences 26(1):37-40. Dhanya,Lakshmana Reddy D C, Veerae Gowda and Aswath C, 2013, The genetic relatedness analysis of male sterile and their maintainer lines of onion (Allium cepa L.) by using RAPD markers. Journal of Crop science and Biotechnology,.March volume, Springer publications. Kanupriya, M Shobana, C Vasugi, C Aswath, V Radhika, Laxman Reddy and M R Dinesh. 2012.Genetic relationship among papaya (Carica papaya) and wild papaya (Vasconcellea species) using RAPD and ISSR markers. Indian Journal of Agricultural Sciences 82 (4): 368–71. Kanupriya; P. Madhavi Latha; C. Aswatha; Laxman Reddy; B. Padmakara; C. Vasugic; M. R. Dineshc 2011, Cultivar Identification and Genetic Fingerprinting of Guava (Psidium guajava) Using Microsatellite Markers. International Journal of Fruit Science 11:184-196. Taylor and Francis, UK D.C.Lakshmana Reddy, H.H.Kumara swamy , Kanti meena and V.Dinesh Kumar,2009. “Host-pathogen bargaining via molecular signals- A brief review”, J.Oilseeds Res., Vol 26 (Special issue) pp181-8183. Kanti Meena , H.H.Kumara swamy, D.C.Lakshmana Reddy, V.Dinesh Kumar and D.K.Meena, 2009, Gamma linolenic acid (GLA) enhancement through genetic engineering in oilseeds crops: a brief review . J.Oilseeds Res., Vol 26 (Special issue) pp185-188. .H.Kumara swamy, V.Dinesh Kumar D.C.Lakshmana Reddy, Kanti meena , K.Sai kumar, V.Srikanth and B.Madhu, 2009, Single cell oil: can it be supplementary to plant oils in meeting the growing demand? J.Oilseeds Res., Vol 26 (Special issue) pp217-220, 2009. . List of papers presented in conferences • Delivered a Oral presentation on “ Bioinformatics work at IIHR” at National seminar on “Hortinformatics-National consultative meet on Bioinformatics in Horticulture” held at IISR, Calicut held on 11th-12th October 2010. • Delivered a Oral Presentation on “Molecular markers for breeding fungal resistance in horticultural crops” at National seminar on “National symposium on Molecular approaches for management of fungal diseases of crop plants” held at I.I.H.R, Bangalore held on 27-30 December 2010. Delivered a lead presentation on " Role of molecular markers in diseases resistance breeding in horticultural crops" at 10th national symposium on " biotechnological approaches for plant protection: constraints and opportunities held at ICAR research complex for Goa, from Jan 27-29, 2013. Delivered a lead presentation on "Genomics in Horticulture: Present status and future needs" at Global conference on Horticulture for food, Nutrition, and livelihood options held at Bhubaneswar, India from May28-31, 2012. Poster presentation on DNA based Diagnostics for identification of citrus rootstocks cv Rangapur lime, Rough lemon and Pseudo lemon at International conference on plant biotechnology for food security : New Frontiers held at NAAS center, Pusa, NewDelhi from Feb 21-24, 2012 Delivered a invited lecturer on " Development of SCAR markers for specific traits at NRC banana , Trichy during workshop on Theory and Practices on Advances in gene identification and marker development from 1-10 Oct 2012 Poster presentation on "Identification of molecular markers linked to resistance against bacterial wilt ( Ralstonia solanacearum) in eggplant" at 7th International symposium on In vitro culture and horticultural breeding “Biotechnological advances in In vitro Horticultural breeding” at Ghent, Belgium, Europe from 18/09/2011 to 22/09/2011 Book Chapters C.Aswath and Lakshmana Reddy D C., Gerbera and Carnation: Molecular markers and Markers assisted selection: Advances in Horticultural Biotechnology, Vol-IV: Vegetables,ornamentals and tuber crops. Edited by H.P.Singh., V. A. Parthasarthy and Nirmal Babu., Published by Westville Publishing House. New Delhi. C.Aswath and Lakshmana Reddy D C., Gladiolus: Molecular markers and Markers assisted selection: Advances in Horticultural Biotechnology, Vol-IV: Vegetables, ornamentals and tuber crops. Edited by H.P.Singh., V. A. Parthasarthy and Nirmal Babu., Published by Westville Publishing House. New Delhi. C.Aswath and Lakshmana Reddy D C., Lilium: Molecular markers and Markers assisted selection: Advances in Horticultural Biotechnology, Vol-IV: Vegetables, ornamentals and tuber crops. Edited by H.P.Singh., V. A. Parthasarthy and Nirmal Babu., Published by Westville Publishing House. New Delhi.

Awards & Recognitions, Patents, Inventions, etc: 

Selected as a Scientist (Biotechnology) through competitive examination for Agricultural research service scientist (biotechnology) recruitment conducted by ASRB, New Delhi (Topper in merit list) Backward classes and minorities (BCM) residential scholarship in B.Sc(Ag) and M.Sc (Ag), Government of Karnataka, India Patents under process/filed : 1. DNA Based Diagnostics for Identification of Citrus rootstock cultivars Rangpur Lime, Rough Lemon and Galgal( Indian patent) Technologies Developed: • SSR based assay Identification of F1 hybrid of Solanum melongena and Solanum Macroparon • SSR based Hybrid purity assessment assay for Arka Meghana • Multiplex PCR assay for identification citrus rootstocks Development of unique DNA barcodes: Eggplant Varieties- IIHR-3, IIHR-7, Arka Keshava, 2 BMG 1, SMS 6-6

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Contact Details
Email Address: 
lreddy@iihr.ernet.in
Telephone #: 
9448402020

Kanupriya

Designation: 
Scientist (Pomology)
Division/Section: 
Division of Biotechnology
Specialisation: 
Genomics and Molecular Breeding
Qualification: 
PhD.
Date of Birth: 
11/18/1975
Experience (No. of Years): 
4 years
Permanent Address: 
B 11/02, Platinum City, Yeshwantpur
Research Accomplishments: 

Fingerprinting of guava and papaya genotypes Validation of markers in papaya Development of genomic resources in pomegranate

Important Publications: 

1. Genetic relationship among papaya (Carica papaya) and wild papaya (Vasconcellea species) using RAPD and ISSR markers (2012).Kanupriya, M Shobhana, C Vasugi, C Aswath, V Radhika, L Reddy and M R Dinesh. Indian Journal of Agricultural Science, 82 (4): 366–9 2.Cultivar Identification and Genetic Fingerprinting of Guava (Psidium guajava) Using Microsatellite Markers (2011).Kanupriya; P. Madhavi Latha; C. Aswath; Laxman Reddy; B. Padmakar; C. Vasugi; M. R. Dinesh International Journal of Fruit Science, 11: 2, 184 — 196 3. SSR-Based DNA Barcodes as a Tool for Identification of Eggplant Genotypes (2012). Lakshmana Reddy , Kiran Khandagale , Srikanth H Reddy , C. Kanupriya , Aswath Chennareddy & T. H. Singh. International Journal of Vegetable Science, 18:3, 260-271

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Contact Details
Email Address: 
kanu@iihr.ernet.in
Telephone #: 
9740119960

Vageeshbabu, H.S.

Designation: 
Senior Scientist (Agri. Biotechnology)
Division/Section: 
Division of Biotechnology
Specialisation: 
Biotechnology
Qualification: 
Ph. D.
Experience (No. of Years): 
15
Permanent Address: 
Division of Biotechnology
Research Accomplishments: 

• Characterization of phylloplane and rhizosphere microflora. • Molecular investigations in Bacillus thuringiensis. • Development of Bt transgenic eggplant and tomato for genetic resistance to the important pests using Bt genes and Gene Pyramiding. • Plant tissue culture, transformation and Agrobacterium-plant interactions. • Gene Trapping and Functional Genomics in Tomato. • Development of mass rearing technology for brinjal shoot and fruit borer, Leucinodes orbonalis Guenee and tomato fruit borer, Helicoverpa armigera. • Application of nanotechnology in Agriculture. • Biosafety, IPR and Cartagena Protocol on GMOs.

Important Publications: 

1. Vageeshbabu H. S., Kirti P. B. and Chopra V. L. (2000). The delta-9-stearoyl ACP desaturase from Brassica juncea: Cloning of the Bjfab2 gene and characterization. Biochem. Soc. Trans. 28(5): A209. 2. Vageeshbabu S. Hanur, Kirti, P. B. and Chopra, V. L. (2004). The seed-specific oleosin promoter of Brassica juncea: cloning, characterization and identification of the novel and divergent antisense open reading frame. J. Plant Biochem. Biotechnol. 13: 1-4. 3. Vageeshbabu S. Hanur (2004). Genetic transformation of human cells by a soil phytopathogen presents common molecular strategies. Curr. Sci. 87:856-857. 4. Vageeshbabu S. Hanur (2007). Exciting developments in plant stem cell research. Curr. Sci. 92: 1668-1670. 5. Vageeshbabu S. Hanur (2008). Bt resistance and monophagous pests: Handling with prudence. Curr. Sci. 95: 449-451. 6. Vageeshbabu S. Hanur (2011). GM crops and centre of origin- a case study of Bt brinjal in India. Curr. Sci. 100:1285-1286. 7. Vageeshbabu S. Hanur (2011). A plant thermometer gene for measuring climate change. Curr. Sci. 100:1135-1137. 8. Vageeshbabu S. Hanur (2011). Ingenious ways of Bt resistance management. Curr. Sci. 101: 146-148. 9. Vageeshbabu S. Hanur (2011). Bt resistance in Helicoverpa species: Indian policy needs urgent revision. Curr. Sci. 101: 470-471. 10. Vageeshbabu S. Hanur, Reddy, P. V. R., Boopal, K., Srividya, K. N., Poovarasan, S., Bhalewar, S. and Saraswathi, M. S. (2011). Solanum macrocarpon, a wild brinjal, is not a source of resistance against brinjal shoot and fruit borer, Leucinodes orbonalis Guenee. Curr. Sci. 101: 1427-1429. 10

Patents, Inventions, Technology Developed: 
Technology: "Mass rearing of brinjal shoot and fruit borer, Leucinodes orbonalis"
Awards & Recognitions, Patents, Inventions, etc: 

1. Represented the Indian Council of Agricultural Research as the Young Scientist, for the Millennium Youth Parliament, conducted by Nehru Yuva Kendra Sangathan, at Vigyan Bhavan, New Delhi, April 23-24, 2000. 2. Indian Science Congress Association (Millennium Session) “Young Scientist” (Biochemistry, Biophysics and Molecular Biology), 2001. 3. Course Coordinator of Winter School on “Biotechnology in Horticulture”. 4. Rapporteur in Insect Bioinformatics Seminar, Bangalore, Nov. 2008. 5. PG Teacher in the field of Biotechnology at the NRC on Plant Biotechnology, IARI, New Delhi, May 1999. 6. PG Teacher in the field of Biotechnology in University of Agricultural Sciences, Bangalore, September 2004. 7. Conducted the First National Level Contained Limited Open field trials of Bt transgenic crops at Bangalore as approved by the Department of Biotechnology and Ministry of Environment & Forests, New Delhi, 2005. 8. PG Teacher in the field of Biotechnology in Kuvempu University, Karnataka. 9. PG Teacher in the field of Biotechnology in University of Horticultural Sciences, Bagalkot. 10. PG Teacher in the field of Biotechnology in YSR Horticultural University, West Godavari, Andhra Pradesh. 11. Invited lead speaker on “Confluence of opportunities” during CONFLUENCE-08 Seminar Series of the Indian Academy College, Bangalore, 28.2.2008. 12. Acted as Course Coordinator of the Summer School on “Non-conventional approaches for crop improvement in horticultural crops”, organized by the Division of Biotechnology, IIHR, Bangalore, June 16-July 06, 2009 and conducted lectures and practical classes. 13. Rapporteur, Theme area II: Institutional and Policy Support: Socio-Economic Domains; Technical Session: 2.4: Food, Nutrition and Health Security, 2nd International Conference on Horticulture, 9-12.11.2009, Bangalore. 14. Lead invited presentation on “Biotechnological interventions in horticulture and sustainable livelihood”, 2nd International Conference on Horticulture, 9-12.11.2009, Bangalore. 15. Acted as Rapporteur in the Technical Session on “Transgenics” and member, Publication Committee, in the National Seminar on Horticultural Biotechnology, held in IIHR, Bangalore, on 28-29, October 2009. 16. Editor, International Journal of Life Sciences, Jaipur. 17. Member, Institute Biosafety Committee under Department of Biotechnology, New Delhi. 18. Subject matter expert of the special training program for officers of Department of Agriculture, Government of Kerala (15.5.2007-22.5.2007). 19. Trainer on GM crops during the Special Training Program for the Officers of Department of Agriculture, Government of Kerala, held in IIHR, Bangalore, on 04-10.01.2008. 20. Trainer on GM crops during the Special Training Program for the Officers of Department of Agriculture, Kerala, held in IIHR, Bangalore, on 02-09.02.2008. 21. Invited guest speaker on “Challenges of novel GM traits in agriculture” during the Winter School on “Research and Development Needs in Transgenic Farming Era”, University of Agricultural Sciences, Dharwad, 11-31.12.2008. 22. Rapporteur, Transgenics Session, National Seminar on Horticultural Biotechnology, Bangalore, 28-29.10.2009. 23. Invited speaker of the Technical Session of the State level Conference on Genetic Engineering, Farming and Food, at Bangalore, 07.11.2009. 24. Trainer on GM crops during the Special Training Program and Interactive Meeting for the field executives of United Phosphorous Ltd. Officers, held in IIHR, Bangalore, on 20-21 October, 2009. 25. Presented an Invited talk on Bt Brinjal in the Seminar on GM crops organized by the Institution of Engineers, Mysore Chapter, on November 2009. 26. Invited speaker and convener delivering key note address on “Designer horticultural crops” at the National Seminar on New Biology and Plant Engineering, organized by School of Life Sciences, Jaipur National University, Jaipur, 01-02.12.2009. 27. Represented IIHR for the Bt brinjal Meeting organized by Director-General, ICAR and Secretary, DARE, Ministry of Agriculture, Krishi Bhavan, New Delhi, 22.01.2010. 28. Invited speaker of the Consultative Meeting cum Workshop, organized by Karnataka Biodiversity Board on GM crops, 26.5.2010. 29. Represented IIHR in the meeting of Biotechnologists, organized by the Foundation for Biotechnology Awareness and Education, for a representation to the Government of India on rescinding of the moratorium on GM crops, July 2010. 30. Served as Honorary Referee of Karnataka Journal of Agricultural Sciences during 2010-11.

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Contact Details
Email Address: 
vageesh@iihr.ernet.in
Telephone #: 
91-80-28466420 Extn: 401

Asokan R.

Designation: 
Principal Scientist (Agricultural Entomology)
Division/Section: 
Division of Biotechnology
Specialisation: 
RNAi in insect pest management; DNA Barcode and Molelcular Phylogeny; Coleoptera specific Bt
Qualification: 
Ph.D.
Experience (No. of Years): 
20
Permanent Address: 
IIHR
Research Accomplishments: 

Potential of RNAi in the management of the boll worm, Helicoverpa armigera has been validated

Important Publications: 

1. R. Asokan, K. B. Rebijith, Sakthi K. Singh, A. S. Sidhu, S. Siddharthan, Praveen K. Karanth, R. Ellango and V. V. Ramamurthy. 2011. Molecular identification and phylogeny of Bacterocera species (Diptera: Tephritidae). Florida Entomologist, 94:1026-1035.

2. H. M. Mahadevaswmy, R. Asokan, S. N. Nagesha, D. K. Arora, Ajantha Birah and Riaz Mahmood. 2011. Cloning, characterization and diversity of insecticidal crystal protein genes of Bacillus thuringiensis native isolates from the soils of Andaman & Nicobar Islands. Current Microbiology, 63: 420-425.

3. R. Asokan, K. B. Rebijith, N. K. Krishna Kumar, K. K. Srikumar, P. Sivarama Bhat. 2011. Development of DNA barcode and species-specific markers for Helopeltis antonii Signoret & Pachypeltis maesarum (Kirkaldy) (Heteroptera: Miridae), pests of Cashew (Anacardium occidentale L). Entomological News, 122: 173-181.

4. K. B. Rebijith, R. Asokan and N. K. Krishna Kumar. 2011. Development of species- specific markers and molecular differences in mtDNA of Thrips palmi Karny and Scirtothrips dorsalis Hood (Thripidae: Thysanoptera), vectors of tospoviruses (Bunyaviridae) in India. Entomological News, 122: 201-213.

5. M. Manamohan, M.N. Prakash, G. Sharath Chandra, R. Asokan and S. N. Nagesha. 2011. An improved protocol for rapid and efficient Agrobacterium mediated transformation of tomato (Solanum lycopersicum L.). Journal of Applied Horticulture, 13:3-7.

6. R. Asokan, H. M. Mahadevaswamy and D. K. Arora.2012. Screening, Diversity and Partial Sequence Comparison of Vegetative Insecticidal Protein (vip3A) Genes in the Local Isolates of Bacillus thuringiensis Berliner. Current Microbiology, 64: 365-370.

7. R. Asokan, S. N. Nagesha, M. Manamohan, N. K. Krishnakumar, H. M. Mahadevaswamy,M. N. Prakash, G. Sharath Chandra, K. B. Rebijith and R. Ellango.2012. Common siRNAs for various target genes of the fruit borer, Helicoverpa armigera Hubner (Lepidoptera: Noctuidae). Current Science, 102: 1692-1699.

8. R.Asokan, S.N. Nagesha, M. Manamohan, N.K. Krishna kumar, H.M. Mahadevaswamy, K.B. Rebijith, M.N. Prakash and G.Sharath Chandra. 2012. Molecular diversity of Helicoverpa armigera Hubner (Noctuidae: Lepidoptera) in India. Oriental Insects, 46: 130-143.

9. R. Asokan, K. B. Rebijith, N. K. Krishna Kumar, K. K. Srikumar, P. Shivarama Bhat and V. V. Ramamurthy. 2012. Molecular identification and diversity of Helopeltis antonii and Helopeltis theivora (Hemiptera: Miridae) in India. Florida Entomologist, 94:350-358.

10. G. Sharath Chandra, R. Asokan, M. Manamohan. 2012. Modified competing polymerase chain reaction primer for single tube quantitative PCR. Analytical Biochemistry, 427:175–177.

11. Shalini Thakur Singh, Natarajan Gayatri Priya, Jitendra Kumar, Vipin Singh Rana, R. Ellango, Adita Joshi, Garima Priyadarshini, R. Asokan, Raman Rajagopal. 2012. Diversity and phylogenetic analysis of endosymbiotic bacteria from field caught Bemisia tabaci from different locations of North India based on 16S rDNA library screening. Infection, Genetics and Evolution, 12: 411–419.

12. K. B. Rebijith, R. Asokan, N. K. Krishna Kumar, V. V. Ramamurthy, V. Krishna. 2012. Development of species-specific markers and molecular differences of Aphis gossypii Glover and Myzus persicae (Sulzer), vectors of Potyviruses (Potyviridae). Florida Entomologist, 95: 677-685.

13. H. K. Roopa, N. K. Krishna Kumar, R. Asokan, K. B. Rebijith, Riaz Mahmood and Abraham Verghese. 2012. Phylogenetic analysis of Trialeurodes spp. (Hemiptera: Aleyrodidae) from India based on differences in mitochondrial and nuclear DNA. Florida Entomologist, 95: 1086-1094.

14. K. B. Rebijith, R. Asokan, N. K. Krishna Kumar, K. K. Sreekumar, V. V. Ramamurthy & P. Shivarama Bhat. 2012. DNA barcoding and development of species-specific markers for the identification of tea mosquito bugs (Miridae: Heteroptera) in India. Environmental Entomology, 41: 1239-1245.

Patents, Inventions, Technology Developed: 
Single step method for ihp assembly in plants for RNAi
Awards & Recognitions, Patents, Inventions, etc: 

Single step method for ihp assembly in plants for RNAi

Fellow of AAPMHE, Bangalore

Order: 
88
Contact Details
Address for Communication: 
Division of Biotechnology, IIHR, Bangalore - 560 089
Email Address: 
asokan@iihr.ernet.in
Telephone #: 
080-28466420/21/22/23 Ext 402

Ravishankar, K.V.

Designation: 
Principal Scientist
Division/Section: 
Division of Biotechnology
Specialisation: 
Genomics and Marker Assisted Selection
Qualification: 
Ph.D (Crop Physiology)
Experience (No. of Years): 
18
Research Accomplishments: 

-Developed microsatellite markers for mango, banana, okra and pomegranate. -Invovled in gene pyarmiding and development of tomato hybrids. -Assessement of genetic diversity and characterization of Indian mango cultivars Projects involved: 1. Functional Genomics of Fusarium wilt and drought tolerance in Banana (ICAR NPTC; as PI) 2. Morphological and Molecular Characterization of Wild and Indigenous Mango Varieties of Indo- Burma Region (North- Eastern Region)(DBT; as PI) 3.Metabolomics of tomato with special reference to fruit quality (DBT; as associate) 4. Development of microsatellite markers in okra and pomegranate

Important Publications: 

Ravishankar, K.V. Lalitha Anand and Dinesh, M.R. 2000 Assesment of genetic relatedness among a few Indian mango varieties using RAPD marker. The Journal of Horticultural Science and Biotechnology. 75:198-201 Shibu M.P., Ravishankar, K.V ., Lalitha Anand, Ganeshaiah K.N. and Uma Shaanker, R. 2000 Identification of sex specific DNA markers in the dioecious tree nutmeg ( Myristica fragrans Houtte). Plant Genetic Resources News Letter. 121:59-61 Ravishankar, K.V. Chandrashekara P. Sreedhar, S.A. Dinesh, M.R. Lalitha Anand and Saiprasad, G.V.S. 2004 Diverse genetic bases of Indian Polyembryonic and Monoembryonic Mango (Mangifera indica L) Cultivars. Current Science. 87:870-71 Vasantaiah HK, Ravishankar KV, Shivashankar KS , Anand L, Narayanaswamy P, Mukunda G and Prasad TG 2006 Cloning and characterization of differentially expressed genes of internal breakdown in mango fruit (Mangifera indica). Journal of Plant Physiology 163:671-679 Ramesha BT, R. Srirama, G. Ravikanth, K. V. Ravishankar, S. Dayanandan,N. Sreejayan,R. Vasudeva, K. N. Ganeshaiah And R. Uma Shaanker 2008 Development of polymorphic microsatellite loci in Nothapodytes nimmoniana, a medicinally important tree from the Western Ghats, India Molecular Ecology Resources 9:365-67 Ravishankar KV, BH. Mani, MR. Dinesh, and Lalitha Anand 2011. Development of new microsatellite markers from Mango (Mangifera indica) and cross-species amplification. American Journal of Botany 98(4):e96-9. doi:10.3732/ajb.1000263 Ravishankar KV, A Rekha, V Swarupa, and G Savitha 2011 Gene Expression analysis in roots of Musa acuminata ssp. burmannicoides ‘Calcutta-4’, a Banana genotype tolerant to Fusarium wilt. Acta Horticulturae. 897:363-370 Ravishankar KV, Rekha A, Malarvizhi, M., Ambika, DS., 2011. Apomictic seed development in Ensete superbum induced by pollen ofwild banana sp. Musa balbisiana. Current Science 10:494-495 Ravishankar KV, L. Vidhya, A. Cyriac, A. Rekha, R. Goel, N.K. Singh and T.R. Sharma 2012 Development of SSR markers based on a survey of genomic sequences and their molecular analysis in banana (Musa spp.) Journal of Horticultural Science and Biotechnology, 87:84-88 Vasugi, C., M. R. Dinesh, K. Sekar,K. S. Shivashankara, B. Padmakar and K. V. Ravishankar 2012 Genetic diversity in unique indigenous mango accessions (Appemidi) of the Western Ghats for certain fruit Characteristics Current Science 103: 199-207 Khadke, G. N.; Hima Bindu, K.; Ravishankar, K. V. 2012 Development of SCAR marker for sex determination in dioecious betelvine (Piper betle L.) Current Science 103:712-716

Important Publications: 
Ravishankar, K.V. Lalitha Anand and Dinesh, M.R. 2000 Assesment of genetic relatedness among a few Indian mango varieties using RAPD marker. The Journal of Horticultural Science and Biotechnology. 75:198-201
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Contact Details
Email Address: 
kvravi@iihr.ernet.in;kv_ravishankar@yahoo.co.in
Telephone #: 
28466420 ext 406

Pious Thomas

Designation: 
Principal Scientist (Horticulture)
Division/Section: 
Division of Biotechnology
Specialisation: 
Biotechnology (Endophytic & Mol. Microbiology)
Qualification: 
Ph.D; Post-Doctorate
Date of Birth: 
12/01/1960
Experience (No. of Years): 
24 yrs
Permanent Address: 
No. 1, Park Place, Baptist Hospital Campus, Hebbal, Bangalore -560024
Research Accomplishments: 

TECHNOLOGIES DEVELOPED  Micropropagation technology for (i) triploid seedless watermelon, (ii) grapes and (iii) chrysanthemum  Sachet technique for the small-scale acclimatization of micropropagated plants of different crops  Diagnostics for the detection of covert and endophytic bacteria in tissue cultures CONCEPTS  Role of leaf in promotion of adventitious rooting; Identification and cloning of genes involved in regulation of rooting in stem cuttings  Identification of covert and unsuspecting association of diverse bacteria as the cause for the decline in the performance or degeneration of micropropagated cultures  Cleansing the established in vitro cultures from covert microbial contaminants through a combination of surface disinfection, antibiotic challenge followed by recurrent tissue indexing  Identification of normally unsuspected routes of microbial entry in plant tissue cultures including deep seated endophytes, alcohol resistant bacteria and autoclaving defying spore-formers, and identification of 90% ethanol as a universally adoptable better bacteri/sporicidal level of alcohol  Establishing the ubiquitous and integral association of endophytes with plant tissue in no-cultivable form through molecular and microscopic techniques  Isolation and characterization of a series of bacterial endophytes from different plant hosts including field grown plants and in vitro stocks  Demonstration of abundant bacterial colonization in the peri-space between cell wall and plasma membrane in banana, and revelation of cytoplasmic peri-space as a niche for endophytic bacteria  Elucidation of the chances of bacterial cell injury due to the inoculum spreader during spread plating and development of a simple spotting-and-tilt-spreading (SATS) to maximize CFU and to avoid CFU inconsistencies during bacterial sampling.

Important Publications: 

1. Thomas, P., Mujawar, M. M., Upreti, R., Sekhar, A. C. 2013. Improved recovery of Bacillus spores from nonporous surfaces with cotton swabs over foam, nylon, or polyester, and the role of hydrophilicity of cotton in governing the recovery efficiency. Applied and Environmental Microbiology. 79: 381-384. 2. Thomas, P., Sekhar, AC., Mujawar, MM. 2012. Non-recovery of varying proportions of viable bacteria during spread-plating governed by the extent of spreader usage and proposal for an alternate spotting-spreading approach to maximize the CFU. Journal of Applied Microbiology 113: 339-350 3. Thomas, P. 2012. Long-term survival of Bacillus spores in alcohol and identification of 90% ethanol as relatively more spori/bactericidal. Current Microbiology 64: 130-139 4. Thomas, P. 2011. Intense association of non-culturable endophytic bacteria with antibiotic-cleansed in vitro watermelon and their activation in degenerating cultures. Plant Cell Reports 30: 2313-2325 5. Thomas, P. and Kumari, S. 2010 Inconspicuous endophytic bacteria mimicking latex exudates in shoot-tip cultures of papaya. Scientia Horticulturae. 124: 469-474 6. Thomas, P., Soly, T. A. 2009. Endophytic bacteria associated with growing shoot tips of banana (Musa sp.) cv. Grand Naine and the affinity of endophytes to the host. Microbial Ecology 58: 952-964 7. Thomas P., Swarna GK, Patil P and Rawal R. D. 2008. Ubiquitous presence of normally non-culturable endophytic bacteria in field shoot-tips of banana and their gradual activation to quiescent cultivable form in tissue cultures. Plant Cell Tissue Organ Culture 93:39-54 8. Thomas, P., Kumari, S., Swarna, G.K. and Gowda, T.K.S. 2007. Papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host-endophyte interaction in vitro and in vivo. Canadian Journal of Microbiology 53: 380-390 9. Thomas, P. 2006. Isolation of an ethanol-tolerant endospore-forming Gram-negative Brevibacillus sp as a covert contaminant in grape tissue cultures. Journal of Applied Microbiology 101: 764-774 10. Thomas, P. 2004. In vitro decline in plant cultures: Detection of a legion of covert bacteria as the cause for degeneration of long-term micropropagated triploid watermelon cultures. Plant Cell Tissue and Organ Culture 77: 173-179

Awards & Recognitions, Patents, Inventions, etc: 

 Fellow, National Academy of Agricultural Sciences, New Delhi (from 1st Jan. 2011)  Shri. Hari Krishna Shasri Memorial Award (2011) from IARI, New Delhi in Feb. 2012 for research contributions in the area of Molecular Profiling and Endophytic Microbiology  Shri. Giridhari Lal Chadha Memorial Gold Medal (2010), from Horticultural Society of India New Delhi.  DBT Overseas Associateship (Short-term) Award (2006-07) – 6 months (March-Sept. 2007). Availed at the Flinders University, Adelaide, Australia  Sukumar Basu Memorial Award (1999-2000) conferred by IARI, New Delhi in Feb. 2002 for research contributions in the area of Horticultural Biotechnology  DBT, Overseas Long term Associateship (Long-term) Award (1999-2000) availed at the University of Michigan, Ann Arbor, USA (1½ yrs)  Commonwealth Post-Doctoral Fellowship awarded during 1989-90 for advanced research at the University of Sheffield, Sheffield, U. K. (1+ year) 1989-1990)  Young Scientist Award at Kerala Science Congress 1990  Best Poster Award from Horticultural Society of India, New Delhi  Fellow: Horticultural Society of India, New Delhi (From Nov. 2010)  Fellow, Plant Tissue Culture Association of India (PTCA-I), New Delhi (From Feb 2004)

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Contact Details
Email Address: 
pioust@iihr.ernet.in
Telephone #: 
28466420 ext 410

Mythili, J.B.

Designation: 
Principal Scientist
Division/Section: 
Division of Biotechnology
Specialisation: 
Tissue culture, Genetic engineering, Transgenics for fungal resistance
Qualification: 
Ph.D
Date of Birth: 
09/22/1963
Permanent Address: 
G2, Prashanth Apts, 5th main, 5th Cross, CPV Block, Ganganagar, Bangalore-560032
Research Accomplishments: 

1. Protocols for large scale propagation of pointed gourd, hybrids of hippeastrum, tuberose and male sterile lines of cauliflower have been developed. Established embryogenic cultures from anthers of Capsisum for development of haploids.

2. Optimization of in vitro regeneration protocols for transgenosis was accomplished in tomato, chilli, onion and carnation.
3. The transgenic lines of tomato and chilli with chitinase gene have shown delayed and moderately reduced disease development as compared to control and preliminary studies with transgenic lines of tomato with PGIP gene have shown promise against late blight. Keeping this in view, pyramiding of tomato with multiple transgenes is being done using double or triple gene constructs for enhanced fungal resistance.

Important Publications: 

1. Mythili, J.B, Rajeev, P.R, Shailesh Yadav, Ramanjini Gowda, P.H, Girija Ganeshanand Chowdappa, P (2012). Stable integration and expression of chitinase gene in transgenic tomato . Acta Horticulturae 929: 373-379

2. Mythili, J.B, Rajeev, P.R and Chethana, B.S (2012). Factors influencing in vitro regeneration and Agrobacterium mediated transformation of onion cv Arka Niketan” Acta Horticulturae 929: 417-422

3. J B Mythili,G V S Saiprasad, C Naveena, P R Rajeev & K K Upreti (2011) Differential response of tomato and tobacco to Agrobacterium mediated transformation with cytokinin independent -1 ( CKI-1) gene as influenced by cytokinin levels. Indian Journal of experimental biology, 49: 909-918.

4. M. Kallesh Prasad, J.B. Mythili, Tejaswini, Lalitha Anand, H.J. Rashmi and C. Suneetha 2009. Optimization of regeneration protocol and Agrobacterium mediated transformation in carnation (Dianthus caryophyllus L.) J Hort sci. 4: 120-127

5. Saiprasad, GVS., Mythili, JB., Lalitha Anand, Suneetha, C, Rashmi, HJ, Naveena, C and Girija Ganeshan.(2008). Development of a Trtichoderma harzianum endochitinase gene construct conferring antifungal activity in transgenic tobacco. Indian Journal of Biotechnology . 8:199-206

6. Shivkumar,B, Mythili, J.B, Lalitha Anand and Saiprasad, G.V.S. 2007. Influence of genotype on Agrobacterium mediated transformation of tomato, Indian J Hort. 64 (3) 251-257

7. Mythili, J.B and Pious Thomas (2003) Effect of stress on induction of androgenic embryos in Capsicum annuum L. cv Arka Gaurav . J plant Biol. 30(3): 293-297

8. Shivegowda, ST, Mythili ,JB, Lalitha Anand, Saiprasad, GVS, Ramanjini Gowda and Gowda, TKS (2002) In vitro regeneration and transformation of chilli pepper (Capsicum annuum L.) J. Hort sci and Biotechnology, 77 (5)629-634.

9. Mythili, J. B. and Thomas, P. 1999. In vitro multiplication of pointed gourd (Trichosanthes dioica Roxb.) Scientia Horticulturae 79: 87-90.

10. Mythili, J. B. and Thomas, P. 1999. Influence of ammonium to nitrate nitrogen ratio in different basal media on callusing response of capsicum (Capsicum annuum L. grossum Sendt.) anthers, Indian Journal of Experimental Biology 37: 314-316.

Awards & Recognitions, Patents, Inventions, etc: 

Technology for development of micropropagation protocol in pointed gourd, tuberose, hippeastrum and male sterile lines of cauliflower was necessitated by the evergrowing demand and the need for disease free planting material. Further, the adoption of micropropagation technology is restricted by the non-availability of protocols to small scale entrepreneurs. The chitinase gene construct developed in house has been taken by few ICAR institutes and Universities

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Contact Details
Address for Communication: 
G2, Prashanth Apts, 5th main, 5th Cross, CPV Block, Ganganagar, Bangalore-560032
Email Address: 
jbm@iihr.ernet.in

Leela Sahijram

Designation: 
Principal Scientist
Division/Section: 
Division of Biotechnology
Specialisation: 
Plant tissue culture systems: hybrid embryo rescue, somatic embryogenesis, micropropagation and specific-pathogen free plantlet production using micrografting/meristem culture
Qualification: 
Ph.D.
Experience (No. of Years): 
31 years
Permanent Address: 
42/1, GF, Deepak Apts., IV Main SBM Colony, V.G. Layout, Anand nagar, Hebbal, BANGALORE 560 024
Research Accomplishments: 

* Team Member in Pioneering work on banana micropropagation in 1980s (including over 20 choice clones from all across India besides Cavendish Group cultivars and Nanjangud Rasabale syn. Rasthali - 'Silk' Group) * Banana Bunchy Top Virus-free and Fusarium Wilt-free banana plantlet production through 'meristem' culture * Hybrid embryo rescue in intergeneric/interspecific/intervarietal crosses in horticultural crops, viz., mango, papaya, seedless grape/lime, banana * Embryo culture in vegetable crops - capsicum, chilli, onion, tomato, okra * Micrografting in citrus * Somatic embryogenesis in mango, pomegranate * Micropropagation: Salt-tolerant grape rootstocks Dogridge, Saltcreek, Ramsey; Passion fruit hybrid Kaveri; Bougainvillea, Chrysanthemum, Carnation, Rose, Lilies; High-value hybrids of tomato and cabbage

Important Publications: 

* Dore Swamy, R. and Leela Sahijram. 1988. Tissue culture propagation of bougainvillea. Gartenbauwissenschaft 53(4):174 * Dore Swamy, R. and Leela Sahijram. 1989. Micropropagation of banana from male floral apices cultured in vitro. Scientia Hortic. 40:181 * Dore Swamy, R. and Leela Sahijram. 1991. Micropropagation of salt-tolerant grape rootstock. In Vitro Cell. Dev. Biol. 27(2):135A * Leela Sahijram and Dore Swamy, R. 1991. Hybrid embryo rescue and nucellar embryogeny in monoembryonate mango cultivars. In Vitro Cell. Dev. Biol. 27(2):148A * Pandey, R.M., R. Doreswamy and Leela Sahijram. 1993. Tissue culture propagation of banana. Silver Jubilee Publication of IIHR, Technical Bulletin No. 11, IIHR, Hessaraghatta, Bangalore * D.K. Samuel, K.S. Mohan, K. Jagadish Chandra & Leela Sahijram. 1999. Detection of banana bunchy top virus in micropropagated plants by a PCR generated DNA probe. Pest Mgt. in Hortl. Ecosystems 5(1):32-37 * Leela Sahijram, Jaya R. Soneji and K.T. Bollamma. 2003. Analyzing somaclonal variation in micropropagated bananas (Musa spp.). In Vitro Cellular & Developmental Biol. (Plant) 39:551-556 * Leela Sahijram and V.C. Kanamadi. 2004. In ovulo hybrid embryo culture in controlled grape crosses involving stenospermocarpic parents. Acta Horticulturae 662:281-288 * Leela Sahijram, B.N.S. Murthy & S.D. Shikhamany. 2005. Introgression of downy mildew resistance into grape cv. Thompson Seedless through hybrid embryo rescue in vitro. Ind. J. Hort. 62(2):107-111 * Leela Sahijram, M.R. Dinesh, Jaya R. Soneji, K.T. Bollamma, Y.T.N. Reddy, Y. Sreevalli and Anitha Naren. 2009. Ex vitro shoot-tip grafting for rescuing hybrid vitroplants of mango (Mangifera indica L.) developed through embryo culture. Acta Horticulturae, 820:319-322 * Leela Sahijram, Jaya R. Soneji and Madhugiri Nageswara Rao. 2010. Molecular and genetic characterization of somaclonal variation in micropropagated bananas (Musa spp.). Genes, Genomes and Genomics 4 (Spl. Issue 1):9-17, GSB Journals, USA * Madhugiri Nageshwara Rao, Jaya R. Soneji and Leela Sahijram. 2012. Wild Crop Relatives: Genomic and Breeding Resources -Tropical and Subtropical Fruits, Chapter: Citrus (pp. 43-59), C. Kole (Ed.), Springer-Verlag, Berlin

Patents, Inventions, Technology Developed: 
Technologies developed: Banana micropropagation and specific-pathogen free plantlet production Hybrid embryo rescue in intergeneric/interspecific/intervarietal controlled crosses in mango, banana, seedless grape, seedless lime, papaya; Micrografting in citrus; Micropropagation of salt-tolerant grape rootstocks Dogridge, Saltcreek, Ramsey
Awards & Recognitions, Patents, Inventions, etc: 

Awards & Recognition: * Distinction, M.Sc. Botany (Plant Physiology) 1976, Osmania University, Hyderabad (A.P.) * Best Lecturer Science Faculty, St. Francis Degree College, Secunderabad (A.P.) [Ranked among top 20 Degree Colleges in the country], 1977-78 * Global pioneer Team Member in developing banana micropropagation technology, 1980s * Dr. Vikram Govind Prasad Award 1999-2000 for research * Global pioneer in hybrid embryo rescue in mango, 2000-2001 * Indian pioneer in seedless grape hybrid embryo rescue technology, 2002-03 * Horticultural Society of India Award 2006-07 for research * Rashtriya Sanman Award 2007 for reasearch Technologies developed: * Banana micropropagation and specific-pathogen free plantlet production * Hybrid embryo rescue in intergeneric/interspecific/intervarietal controlled crosses in mango, banana, seedless grape, seedless lime, papaya * Micrografting in citrus * Micropropagation of salt-tolerant grape rootstocks Dogridge, Saltcreek, Ramsey

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Contact Details
Address for Communication: 
Division of Biotechnology, Indian Institute of Horticultural Research (IIHR), Hessaraghatta, BANGALORE 560 089
Email Address: 
leelas@iihr.ernet.in
Telephone #: 
91-80-28466420/21/22/23 Extn. 408

Akella Vani

Designation: 
Principal Scientist (ARS, Genetics and Cell Biology) & I/c. Head
Division/Section: 
Division of Biotechnology
Specialisation: 
Molecular Biology, Gene cloning, Transgenic development, Antibody development
Qualification: 
PhD (USA)
Experience (No. of Years): 
34
Research Accomplishments: 

Developed and released 7 legume varieties Developed transgenic tomato, watermelon, papaya resistant to viruses

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Contact Details
Address for Communication: 
IIHR, Hessaraghatta, Bangalore 560089
Email Address: 
avani@iihr.ernet.in
Telephone #: 
28466420 EXT 405
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