Asokan R.

Designation: 
Principal Scientist (Agricultural Entomology)
Division/Section: 
Division of Biotechnology
Specialisation: 
RNAi in insect pest management; DNA Barcode and Molelcular Phylogeny; Coleoptera specific Bt
Qualification: 
Ph.D.
Experience (No. of Years): 
20
Permanent Address: 
IIHR
Research Accomplishments: 

Potential of RNAi in the management of the boll worm, Helicoverpa armigera has been validated

Important Publications: 

1. R. Asokan, K. B. Rebijith, Sakthi K. Singh, A. S. Sidhu, S. Siddharthan, Praveen K. Karanth, R. Ellango and V. V. Ramamurthy. 2011. Molecular identification and phylogeny of Bacterocera species (Diptera: Tephritidae). Florida Entomologist, 94:1026-1035.

2. H. M. Mahadevaswmy, R. Asokan, S. N. Nagesha, D. K. Arora, Ajantha Birah and Riaz Mahmood. 2011. Cloning, characterization and diversity of insecticidal crystal protein genes of Bacillus thuringiensis native isolates from the soils of Andaman & Nicobar Islands. Current Microbiology, 63: 420-425.

3. R. Asokan, K. B. Rebijith, N. K. Krishna Kumar, K. K. Srikumar, P. Sivarama Bhat. 2011. Development of DNA barcode and species-specific markers for Helopeltis antonii Signoret & Pachypeltis maesarum (Kirkaldy) (Heteroptera: Miridae), pests of Cashew (Anacardium occidentale L). Entomological News, 122: 173-181.

4. K. B. Rebijith, R. Asokan and N. K. Krishna Kumar. 2011. Development of species- specific markers and molecular differences in mtDNA of Thrips palmi Karny and Scirtothrips dorsalis Hood (Thripidae: Thysanoptera), vectors of tospoviruses (Bunyaviridae) in India. Entomological News, 122: 201-213.

5. M. Manamohan, M.N. Prakash, G. Sharath Chandra, R. Asokan and S. N. Nagesha. 2011. An improved protocol for rapid and efficient Agrobacterium mediated transformation of tomato (Solanum lycopersicum L.). Journal of Applied Horticulture, 13:3-7.

6. R. Asokan, H. M. Mahadevaswamy and D. K. Arora.2012. Screening, Diversity and Partial Sequence Comparison of Vegetative Insecticidal Protein (vip3A) Genes in the Local Isolates of Bacillus thuringiensis Berliner. Current Microbiology, 64: 365-370.

7. R. Asokan, S. N. Nagesha, M. Manamohan, N. K. Krishnakumar, H. M. Mahadevaswamy,M. N. Prakash, G. Sharath Chandra, K. B. Rebijith and R. Ellango.2012. Common siRNAs for various target genes of the fruit borer, Helicoverpa armigera Hubner (Lepidoptera: Noctuidae). Current Science, 102: 1692-1699.

8. R.Asokan, S.N. Nagesha, M. Manamohan, N.K. Krishna kumar, H.M. Mahadevaswamy, K.B. Rebijith, M.N. Prakash and G.Sharath Chandra. 2012. Molecular diversity of Helicoverpa armigera Hubner (Noctuidae: Lepidoptera) in India. Oriental Insects, 46: 130-143.

9. R. Asokan, K. B. Rebijith, N. K. Krishna Kumar, K. K. Srikumar, P. Shivarama Bhat and V. V. Ramamurthy. 2012. Molecular identification and diversity of Helopeltis antonii and Helopeltis theivora (Hemiptera: Miridae) in India. Florida Entomologist, 94:350-358.

10. G. Sharath Chandra, R. Asokan, M. Manamohan. 2012. Modified competing polymerase chain reaction primer for single tube quantitative PCR. Analytical Biochemistry, 427:175–177.

11. Shalini Thakur Singh, Natarajan Gayatri Priya, Jitendra Kumar, Vipin Singh Rana, R. Ellango, Adita Joshi, Garima Priyadarshini, R. Asokan, Raman Rajagopal. 2012. Diversity and phylogenetic analysis of endosymbiotic bacteria from field caught Bemisia tabaci from different locations of North India based on 16S rDNA library screening. Infection, Genetics and Evolution, 12: 411–419.

12. K. B. Rebijith, R. Asokan, N. K. Krishna Kumar, V. V. Ramamurthy, V. Krishna. 2012. Development of species-specific markers and molecular differences of Aphis gossypii Glover and Myzus persicae (Sulzer), vectors of Potyviruses (Potyviridae). Florida Entomologist, 95: 677-685.

13. H. K. Roopa, N. K. Krishna Kumar, R. Asokan, K. B. Rebijith, Riaz Mahmood and Abraham Verghese. 2012. Phylogenetic analysis of Trialeurodes spp. (Hemiptera: Aleyrodidae) from India based on differences in mitochondrial and nuclear DNA. Florida Entomologist, 95: 1086-1094.

14. K. B. Rebijith, R. Asokan, N. K. Krishna Kumar, K. K. Sreekumar, V. V. Ramamurthy & P. Shivarama Bhat. 2012. DNA barcoding and development of species-specific markers for the identification of tea mosquito bugs (Miridae: Heteroptera) in India. Environmental Entomology, 41: 1239-1245.

Patents, Inventions, Technology Developed: 
Single step method for ihp assembly in plants for RNAi
Awards & Recognitions, Patents, Inventions, etc: 

Single step method for ihp assembly in plants for RNAi

Fellow of AAPMHE, Bangalore

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Contact Details
Address for Communication: 
Division of Biotechnology, IIHR, Bangalore - 560 089
Email Address: 
asokan@iihr.ernet.in
Telephone #: 
080-28466420/21/22/23 Ext 402

Ravishankar, K.V.

Designation: 
Principal Scientist
Division/Section: 
Division of Biotechnology
Specialisation: 
Genomics and Marker Assisted Selection
Qualification: 
Ph.D (Crop Physiology)
Experience (No. of Years): 
18
Research Accomplishments: 

-Developed microsatellite markers for mango, banana, okra and pomegranate. -Invovled in gene pyarmiding and development of tomato hybrids. -Assessement of genetic diversity and characterization of Indian mango cultivars Projects involved: 1. Functional Genomics of Fusarium wilt and drought tolerance in Banana (ICAR NPTC; as PI) 2. Morphological and Molecular Characterization of Wild and Indigenous Mango Varieties of Indo- Burma Region (North- Eastern Region)(DBT; as PI) 3.Metabolomics of tomato with special reference to fruit quality (DBT; as associate) 4. Development of microsatellite markers in okra and pomegranate

Important Publications: 

Ravishankar, K.V. Lalitha Anand and Dinesh, M.R. 2000 Assesment of genetic relatedness among a few Indian mango varieties using RAPD marker. The Journal of Horticultural Science and Biotechnology. 75:198-201 Shibu M.P., Ravishankar, K.V ., Lalitha Anand, Ganeshaiah K.N. and Uma Shaanker, R. 2000 Identification of sex specific DNA markers in the dioecious tree nutmeg ( Myristica fragrans Houtte). Plant Genetic Resources News Letter. 121:59-61 Ravishankar, K.V. Chandrashekara P. Sreedhar, S.A. Dinesh, M.R. Lalitha Anand and Saiprasad, G.V.S. 2004 Diverse genetic bases of Indian Polyembryonic and Monoembryonic Mango (Mangifera indica L) Cultivars. Current Science. 87:870-71 Vasantaiah HK, Ravishankar KV, Shivashankar KS , Anand L, Narayanaswamy P, Mukunda G and Prasad TG 2006 Cloning and characterization of differentially expressed genes of internal breakdown in mango fruit (Mangifera indica). Journal of Plant Physiology 163:671-679 Ramesha BT, R. Srirama, G. Ravikanth, K. V. Ravishankar, S. Dayanandan,N. Sreejayan,R. Vasudeva, K. N. Ganeshaiah And R. Uma Shaanker 2008 Development of polymorphic microsatellite loci in Nothapodytes nimmoniana, a medicinally important tree from the Western Ghats, India Molecular Ecology Resources 9:365-67 Ravishankar KV, BH. Mani, MR. Dinesh, and Lalitha Anand 2011. Development of new microsatellite markers from Mango (Mangifera indica) and cross-species amplification. American Journal of Botany 98(4):e96-9. doi:10.3732/ajb.1000263 Ravishankar KV, A Rekha, V Swarupa, and G Savitha 2011 Gene Expression analysis in roots of Musa acuminata ssp. burmannicoides ‘Calcutta-4’, a Banana genotype tolerant to Fusarium wilt. Acta Horticulturae. 897:363-370 Ravishankar KV, Rekha A, Malarvizhi, M., Ambika, DS., 2011. Apomictic seed development in Ensete superbum induced by pollen ofwild banana sp. Musa balbisiana. Current Science 10:494-495 Ravishankar KV, L. Vidhya, A. Cyriac, A. Rekha, R. Goel, N.K. Singh and T.R. Sharma 2012 Development of SSR markers based on a survey of genomic sequences and their molecular analysis in banana (Musa spp.) Journal of Horticultural Science and Biotechnology, 87:84-88 Vasugi, C., M. R. Dinesh, K. Sekar,K. S. Shivashankara, B. Padmakar and K. V. Ravishankar 2012 Genetic diversity in unique indigenous mango accessions (Appemidi) of the Western Ghats for certain fruit Characteristics Current Science 103: 199-207 Khadke, G. N.; Hima Bindu, K.; Ravishankar, K. V. 2012 Development of SCAR marker for sex determination in dioecious betelvine (Piper betle L.) Current Science 103:712-716

Important Publications: 
Ravishankar, K.V. Lalitha Anand and Dinesh, M.R. 2000 Assesment of genetic relatedness among a few Indian mango varieties using RAPD marker. The Journal of Horticultural Science and Biotechnology. 75:198-201
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Contact Details
Email Address: 
kvravi@iihr.ernet.in;kv_ravishankar@yahoo.co.in
Telephone #: 
28466420 ext 406

Pious Thomas

Designation: 
Principal Scientist (Horticulture)
Division/Section: 
Division of Biotechnology
Specialisation: 
Biotechnology (Endophytic & Mol. Microbiology)
Qualification: 
Ph.D; Post-Doctorate
Date of Birth: 
12/01/1960
Experience (No. of Years): 
24 yrs
Permanent Address: 
No. 1, Park Place, Baptist Hospital Campus, Hebbal, Bangalore -560024
Research Accomplishments: 

TECHNOLOGIES DEVELOPED  Micropropagation technology for (i) triploid seedless watermelon, (ii) grapes and (iii) chrysanthemum  Sachet technique for the small-scale acclimatization of micropropagated plants of different crops  Diagnostics for the detection of covert and endophytic bacteria in tissue cultures CONCEPTS  Role of leaf in promotion of adventitious rooting; Identification and cloning of genes involved in regulation of rooting in stem cuttings  Identification of covert and unsuspecting association of diverse bacteria as the cause for the decline in the performance or degeneration of micropropagated cultures  Cleansing the established in vitro cultures from covert microbial contaminants through a combination of surface disinfection, antibiotic challenge followed by recurrent tissue indexing  Identification of normally unsuspected routes of microbial entry in plant tissue cultures including deep seated endophytes, alcohol resistant bacteria and autoclaving defying spore-formers, and identification of 90% ethanol as a universally adoptable better bacteri/sporicidal level of alcohol  Establishing the ubiquitous and integral association of endophytes with plant tissue in no-cultivable form through molecular and microscopic techniques  Isolation and characterization of a series of bacterial endophytes from different plant hosts including field grown plants and in vitro stocks  Demonstration of abundant bacterial colonization in the peri-space between cell wall and plasma membrane in banana, and revelation of cytoplasmic peri-space as a niche for endophytic bacteria  Elucidation of the chances of bacterial cell injury due to the inoculum spreader during spread plating and development of a simple spotting-and-tilt-spreading (SATS) to maximize CFU and to avoid CFU inconsistencies during bacterial sampling.

Important Publications: 

1. Thomas, P., Mujawar, M. M., Upreti, R., Sekhar, A. C. 2013. Improved recovery of Bacillus spores from nonporous surfaces with cotton swabs over foam, nylon, or polyester, and the role of hydrophilicity of cotton in governing the recovery efficiency. Applied and Environmental Microbiology. 79: 381-384. 2. Thomas, P., Sekhar, AC., Mujawar, MM. 2012. Non-recovery of varying proportions of viable bacteria during spread-plating governed by the extent of spreader usage and proposal for an alternate spotting-spreading approach to maximize the CFU. Journal of Applied Microbiology 113: 339-350 3. Thomas, P. 2012. Long-term survival of Bacillus spores in alcohol and identification of 90% ethanol as relatively more spori/bactericidal. Current Microbiology 64: 130-139 4. Thomas, P. 2011. Intense association of non-culturable endophytic bacteria with antibiotic-cleansed in vitro watermelon and their activation in degenerating cultures. Plant Cell Reports 30: 2313-2325 5. Thomas, P. and Kumari, S. 2010 Inconspicuous endophytic bacteria mimicking latex exudates in shoot-tip cultures of papaya. Scientia Horticulturae. 124: 469-474 6. Thomas, P., Soly, T. A. 2009. Endophytic bacteria associated with growing shoot tips of banana (Musa sp.) cv. Grand Naine and the affinity of endophytes to the host. Microbial Ecology 58: 952-964 7. Thomas P., Swarna GK, Patil P and Rawal R. D. 2008. Ubiquitous presence of normally non-culturable endophytic bacteria in field shoot-tips of banana and their gradual activation to quiescent cultivable form in tissue cultures. Plant Cell Tissue Organ Culture 93:39-54 8. Thomas, P., Kumari, S., Swarna, G.K. and Gowda, T.K.S. 2007. Papaya shoot tip associated endophytic bacteria isolated from in vitro cultures and host-endophyte interaction in vitro and in vivo. Canadian Journal of Microbiology 53: 380-390 9. Thomas, P. 2006. Isolation of an ethanol-tolerant endospore-forming Gram-negative Brevibacillus sp as a covert contaminant in grape tissue cultures. Journal of Applied Microbiology 101: 764-774 10. Thomas, P. 2004. In vitro decline in plant cultures: Detection of a legion of covert bacteria as the cause for degeneration of long-term micropropagated triploid watermelon cultures. Plant Cell Tissue and Organ Culture 77: 173-179

Awards & Recognitions, Patents, Inventions, etc: 

 Fellow, National Academy of Agricultural Sciences, New Delhi (from 1st Jan. 2011)  Shri. Hari Krishna Shasri Memorial Award (2011) from IARI, New Delhi in Feb. 2012 for research contributions in the area of Molecular Profiling and Endophytic Microbiology  Shri. Giridhari Lal Chadha Memorial Gold Medal (2010), from Horticultural Society of India New Delhi.  DBT Overseas Associateship (Short-term) Award (2006-07) – 6 months (March-Sept. 2007). Availed at the Flinders University, Adelaide, Australia  Sukumar Basu Memorial Award (1999-2000) conferred by IARI, New Delhi in Feb. 2002 for research contributions in the area of Horticultural Biotechnology  DBT, Overseas Long term Associateship (Long-term) Award (1999-2000) availed at the University of Michigan, Ann Arbor, USA (1½ yrs)  Commonwealth Post-Doctoral Fellowship awarded during 1989-90 for advanced research at the University of Sheffield, Sheffield, U. K. (1+ year) 1989-1990)  Young Scientist Award at Kerala Science Congress 1990  Best Poster Award from Horticultural Society of India, New Delhi  Fellow: Horticultural Society of India, New Delhi (From Nov. 2010)  Fellow, Plant Tissue Culture Association of India (PTCA-I), New Delhi (From Feb 2004)

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Contact Details
Email Address: 
pioust@iihr.ernet.in
Telephone #: 
28466420 ext 410

Mythili, J.B.

Designation: 
Principal Scientist
Division/Section: 
Division of Biotechnology
Specialisation: 
Tissue culture, Genetic engineering, Transgenics for fungal resistance
Qualification: 
Ph.D
Date of Birth: 
09/22/1963
Permanent Address: 
G2, Prashanth Apts, 5th main, 5th Cross, CPV Block, Ganganagar, Bangalore-560032
Research Accomplishments: 

1. Protocols for large scale propagation of pointed gourd, hybrids of hippeastrum, tuberose and male sterile lines of cauliflower have been developed. Established embryogenic cultures from anthers of Capsisum for development of haploids.

2. Optimization of in vitro regeneration protocols for transgenosis was accomplished in tomato, chilli, onion and carnation.
3. The transgenic lines of tomato and chilli with chitinase gene have shown delayed and moderately reduced disease development as compared to control and preliminary studies with transgenic lines of tomato with PGIP gene have shown promise against late blight. Keeping this in view, pyramiding of tomato with multiple transgenes is being done using double or triple gene constructs for enhanced fungal resistance.

Important Publications: 

1. Mythili, J.B, Rajeev, P.R, Shailesh Yadav, Ramanjini Gowda, P.H, Girija Ganeshanand Chowdappa, P (2012). Stable integration and expression of chitinase gene in transgenic tomato . Acta Horticulturae 929: 373-379

2. Mythili, J.B, Rajeev, P.R and Chethana, B.S (2012). Factors influencing in vitro regeneration and Agrobacterium mediated transformation of onion cv Arka Niketan” Acta Horticulturae 929: 417-422

3. J B Mythili,G V S Saiprasad, C Naveena, P R Rajeev & K K Upreti (2011) Differential response of tomato and tobacco to Agrobacterium mediated transformation with cytokinin independent -1 ( CKI-1) gene as influenced by cytokinin levels. Indian Journal of experimental biology, 49: 909-918.

4. M. Kallesh Prasad, J.B. Mythili, Tejaswini, Lalitha Anand, H.J. Rashmi and C. Suneetha 2009. Optimization of regeneration protocol and Agrobacterium mediated transformation in carnation (Dianthus caryophyllus L.) J Hort sci. 4: 120-127

5. Saiprasad, GVS., Mythili, JB., Lalitha Anand, Suneetha, C, Rashmi, HJ, Naveena, C and Girija Ganeshan.(2008). Development of a Trtichoderma harzianum endochitinase gene construct conferring antifungal activity in transgenic tobacco. Indian Journal of Biotechnology . 8:199-206

6. Shivkumar,B, Mythili, J.B, Lalitha Anand and Saiprasad, G.V.S. 2007. Influence of genotype on Agrobacterium mediated transformation of tomato, Indian J Hort. 64 (3) 251-257

7. Mythili, J.B and Pious Thomas (2003) Effect of stress on induction of androgenic embryos in Capsicum annuum L. cv Arka Gaurav . J plant Biol. 30(3): 293-297

8. Shivegowda, ST, Mythili ,JB, Lalitha Anand, Saiprasad, GVS, Ramanjini Gowda and Gowda, TKS (2002) In vitro regeneration and transformation of chilli pepper (Capsicum annuum L.) J. Hort sci and Biotechnology, 77 (5)629-634.

9. Mythili, J. B. and Thomas, P. 1999. In vitro multiplication of pointed gourd (Trichosanthes dioica Roxb.) Scientia Horticulturae 79: 87-90.

10. Mythili, J. B. and Thomas, P. 1999. Influence of ammonium to nitrate nitrogen ratio in different basal media on callusing response of capsicum (Capsicum annuum L. grossum Sendt.) anthers, Indian Journal of Experimental Biology 37: 314-316.

Awards & Recognitions, Patents, Inventions, etc: 

Technology for development of micropropagation protocol in pointed gourd, tuberose, hippeastrum and male sterile lines of cauliflower was necessitated by the evergrowing demand and the need for disease free planting material. Further, the adoption of micropropagation technology is restricted by the non-availability of protocols to small scale entrepreneurs. The chitinase gene construct developed in house has been taken by few ICAR institutes and Universities

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Contact Details
Address for Communication: 
G2, Prashanth Apts, 5th main, 5th Cross, CPV Block, Ganganagar, Bangalore-560032
Email Address: 
jbm@iihr.ernet.in

Leela Sahijram

Designation: 
Principal Scientist
Division/Section: 
Division of Biotechnology
Specialisation: 
Plant tissue culture systems: hybrid embryo rescue, somatic embryogenesis, micropropagation and specific-pathogen free plantlet production using micrografting/meristem culture
Qualification: 
Ph.D.
Experience (No. of Years): 
31 years
Permanent Address: 
42/1, GF, Deepak Apts., IV Main SBM Colony, V.G. Layout, Anand nagar, Hebbal, BANGALORE 560 024
Research Accomplishments: 

* Team Member in Pioneering work on banana micropropagation in 1980s (including over 20 choice clones from all across India besides Cavendish Group cultivars and Nanjangud Rasabale syn. Rasthali - 'Silk' Group) * Banana Bunchy Top Virus-free and Fusarium Wilt-free banana plantlet production through 'meristem' culture * Hybrid embryo rescue in intergeneric/interspecific/intervarietal crosses in horticultural crops, viz., mango, papaya, seedless grape/lime, banana * Embryo culture in vegetable crops - capsicum, chilli, onion, tomato, okra * Micrografting in citrus * Somatic embryogenesis in mango, pomegranate * Micropropagation: Salt-tolerant grape rootstocks Dogridge, Saltcreek, Ramsey; Passion fruit hybrid Kaveri; Bougainvillea, Chrysanthemum, Carnation, Rose, Lilies; High-value hybrids of tomato and cabbage

Important Publications: 

* Dore Swamy, R. and Leela Sahijram. 1988. Tissue culture propagation of bougainvillea. Gartenbauwissenschaft 53(4):174 * Dore Swamy, R. and Leela Sahijram. 1989. Micropropagation of banana from male floral apices cultured in vitro. Scientia Hortic. 40:181 * Dore Swamy, R. and Leela Sahijram. 1991. Micropropagation of salt-tolerant grape rootstock. In Vitro Cell. Dev. Biol. 27(2):135A * Leela Sahijram and Dore Swamy, R. 1991. Hybrid embryo rescue and nucellar embryogeny in monoembryonate mango cultivars. In Vitro Cell. Dev. Biol. 27(2):148A * Pandey, R.M., R. Doreswamy and Leela Sahijram. 1993. Tissue culture propagation of banana. Silver Jubilee Publication of IIHR, Technical Bulletin No. 11, IIHR, Hessaraghatta, Bangalore * D.K. Samuel, K.S. Mohan, K. Jagadish Chandra & Leela Sahijram. 1999. Detection of banana bunchy top virus in micropropagated plants by a PCR generated DNA probe. Pest Mgt. in Hortl. Ecosystems 5(1):32-37 * Leela Sahijram, Jaya R. Soneji and K.T. Bollamma. 2003. Analyzing somaclonal variation in micropropagated bananas (Musa spp.). In Vitro Cellular & Developmental Biol. (Plant) 39:551-556 * Leela Sahijram and V.C. Kanamadi. 2004. In ovulo hybrid embryo culture in controlled grape crosses involving stenospermocarpic parents. Acta Horticulturae 662:281-288 * Leela Sahijram, B.N.S. Murthy & S.D. Shikhamany. 2005. Introgression of downy mildew resistance into grape cv. Thompson Seedless through hybrid embryo rescue in vitro. Ind. J. Hort. 62(2):107-111 * Leela Sahijram, M.R. Dinesh, Jaya R. Soneji, K.T. Bollamma, Y.T.N. Reddy, Y. Sreevalli and Anitha Naren. 2009. Ex vitro shoot-tip grafting for rescuing hybrid vitroplants of mango (Mangifera indica L.) developed through embryo culture. Acta Horticulturae, 820:319-322 * Leela Sahijram, Jaya R. Soneji and Madhugiri Nageswara Rao. 2010. Molecular and genetic characterization of somaclonal variation in micropropagated bananas (Musa spp.). Genes, Genomes and Genomics 4 (Spl. Issue 1):9-17, GSB Journals, USA * Madhugiri Nageshwara Rao, Jaya R. Soneji and Leela Sahijram. 2012. Wild Crop Relatives: Genomic and Breeding Resources -Tropical and Subtropical Fruits, Chapter: Citrus (pp. 43-59), C. Kole (Ed.), Springer-Verlag, Berlin

Patents, Inventions, Technology Developed: 
Technologies developed: Banana micropropagation and specific-pathogen free plantlet production Hybrid embryo rescue in intergeneric/interspecific/intervarietal controlled crosses in mango, banana, seedless grape, seedless lime, papaya; Micrografting in citrus; Micropropagation of salt-tolerant grape rootstocks Dogridge, Saltcreek, Ramsey
Awards & Recognitions, Patents, Inventions, etc: 

Awards & Recognition: * Distinction, M.Sc. Botany (Plant Physiology) 1976, Osmania University, Hyderabad (A.P.) * Best Lecturer Science Faculty, St. Francis Degree College, Secunderabad (A.P.) [Ranked among top 20 Degree Colleges in the country], 1977-78 * Global pioneer Team Member in developing banana micropropagation technology, 1980s * Dr. Vikram Govind Prasad Award 1999-2000 for research * Global pioneer in hybrid embryo rescue in mango, 2000-2001 * Indian pioneer in seedless grape hybrid embryo rescue technology, 2002-03 * Horticultural Society of India Award 2006-07 for research * Rashtriya Sanman Award 2007 for reasearch Technologies developed: * Banana micropropagation and specific-pathogen free plantlet production * Hybrid embryo rescue in intergeneric/interspecific/intervarietal controlled crosses in mango, banana, seedless grape, seedless lime, papaya * Micrografting in citrus * Micropropagation of salt-tolerant grape rootstocks Dogridge, Saltcreek, Ramsey

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Contact Details
Address for Communication: 
Division of Biotechnology, Indian Institute of Horticultural Research (IIHR), Hessaraghatta, BANGALORE 560 089
Email Address: 
leelas@iihr.ernet.in
Telephone #: 
91-80-28466420/21/22/23 Extn. 408

Akella Vani

Designation: 
Principal Scientist (ARS, Genetics and Cell Biology) & I/c. Head
Division/Section: 
Division of Biotechnology
Specialisation: 
Molecular Biology, Gene cloning, Transgenic development, Antibody development
Qualification: 
PhD (USA)
Experience (No. of Years): 
34
Research Accomplishments: 

Developed and released 7 legume varieties Developed transgenic tomato, watermelon, papaya resistant to viruses

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Contact Details
Address for Communication: 
IIHR, Hessaraghatta, Bangalore 560089
Email Address: 
avani@iihr.ernet.in
Telephone #: 
28466420 EXT 405

Dr. Sukhada Mohandas

Designation: 
Emeritus Scientist
Division/Section: 
Division of Biotechnology
Specialisation: 
plant-microbe interaction, development of beneficial microbial strains, Gene cloning ,transformation and transgenic development
Qualification: 
Ph.D
Permanent Address: 
15, Anugraha, II cross, Atmananda Colony, Sultanpalya, Bangalore 560 089. Telephone #: 080 - 284466420 ext. 403 (R) 080-23336774
Research Accomplishments: 

1.Antimicrobial peptide (amp) gene, Plant ferodoxine like protein (Pflp) gene and Defensin genes have been cloned in the laboratory for imparting disease resistance. The cloned genes were expressed in prokaryotic expression vectors. Proteins were purified and tested against number of pathogens for inhibition. Plant transformation vectors were constructed 2. For the development of transgenic Banana resistant to Fusarium wilt methodology was developed first for generating highly regenerative embryogenic cell suspensions (ECS) of the cv Rasthali. One ml of the cell suspension could regenerated 1500-2000 plants. 3. Embryogenic cells of cv Rasthali were transformed with AMP gene and transgenic banana with AMP gene was developed , integration and expression of the gene was confirmed by Southern, ELISA and Western Blot and pot screened for Fusarium resistance. 4. Open field trials were initiated for selecting transgenic lines most torelarant/resistant to Fusarium after obtaining permission from RCGM and Government of Karnataka 5. Micropropagation of banana cultivars Elakki Bale (Neypoovan) and Rasbale (Rasthali) were standardised and the technology is available for industry to adopt. 6. Actinomycetes strains isolated from rhizosphere of fruit crops were molecularly identified as belonging to Streptomyces species and producing antibiotics like neomycin, avermectin and streptomycin. They were found to be having antagonistic effect on plant pathogens including Xanthomonas auxonopodis pv punicae effecting pomegranated. Initial pot trials have revealed that the actinomycetes inhibit Xanthomonas growth and reduce infection in affected plant 7. Important microbial strains of nitrogen fixers, phosphate solubilisers have been developed for use in cultivation of fruits and vegetable crops. Mycorrhizal strains have been identified for inoculation of fruit crops like banana, papaya, pomegranate, mango and citrus plants 8. The role of mycorrhiza in combination with trichoderma and Pseudomonas in imparting disease resistance for Fusarium in banana and Phytophthora in papaya have been elucidated. 9. Molecular markers of been developed for identifying 4 genera of Arbuscular Mycorrhizal fungi and 9 species of Glomus. 10. Allelopathic effects of Parthenium hysterophorus L. operating in nature were studied in detail for the first time(Doctoral work). Allelochemicals released to the environment were purified and their interaction undernatural habitat were studied. Pollen allelopathy, a new concept occurring in nature was put forth for the first time.

Important Publications: 

1. Sukhada Mohandas, Poovarasan, S, Paneerselvam,P, Saritha, B, Upreti, K.K, Ranveer Kamal and Sita, T. 2013. Guava (Psidium guajava L.) rhizosphere Glomus mosseae spores harbor actinomycetes with growth promoting and antifungal attributes . Scientia Horticulture 150 : 371–376. 2. Sukhada Mohandas 2012. Field response of papaya (Carica papaya L. cv. Coorg Honey Dew) to inoculation with arbuscular mycorrhizal fungi at different levels of phosphorus. Journal of Horticultural Science and Biotechnology (2012) 87 (5) 514- 518. 3. Sukhada Mohandas (2012). Arbuscular mycorrhizal fungi benefit mango (Mangifera indica L.) plant growth in the field. Scientia Horticulturae, Volume 143, 43–48. 4.Sukhada Mohandas, R.Manjula, and R.D.Rawal 2011. Evaluation of arbuscular mycorrhiza and other biocontrol agents against Phytophthora parasitica var. nicotianae infecting papaya (Carica papaya cv. Surya) and enumeration of pathogen population using immunotechniques. Biological Control 58 (2011) 22-29. 5.Sukhada Mohandas, R.manjula, R.D.Rawal, H.C.Lakshmikantha, Saikath Chakraborthy, Y.L.Ramachandra. 2010 Evaluation of VAM and other bioagents in managing Fusarium oxysporum f.sp.cubense in banana cb Neypoovan . Biocontrol Science and Technology (UK). 20(2) 165-181. 6. Periyasamy Panneerselvam, Sukada Mohandas, Boya Saritha, Kaushal Kishore Upretti, Poovarasan and Vijay Sulladmath Glomus mosseae associated bacteria andtheir influence on stimulation of mycorrhizal colonization, sporulation and growth promotion in guava (Psidium gujava L.) seedlings. Biological Agriculture and Horticulture iFirst article, 2012, 1–13. 7.Sukhada Mohandas, M. Manamohan, R.D. Rawal, Saikat Chakraborty, H. Sreekantappa, R. Manjula, H.C.Laxmikantha 2004. Interaction of Fusarium Oxysporum f.sp. Cubense with Pseudomonas fluorescens precolonized to Banana Roots. World Journal of Microbiology and Biotechnology 20: 651-655. 8.Sukhada Mohandas 1992. Effect of VAM inoculation on plant growth, nutrient level and root phosphatase activity in papaya (Carica papaya cv Coorg Honey Dew). Fertiliser Research (Netherlands) 31, 263-267. 9.Sukhada Kanchan and Jayachandra 1979. Allelopathic effects of Parthenium hysterophorus L Part 1-IV. Plant and Soil, 53, 27- 35, 37-47, 55: 61-66, 67-75 ( four papers) 11. Sukhada Kanchan and Jayachanadra, 1980. Pollen Allelopathy- A new phenomenon. New Phytologist 84(4), 739-746 12. Sukhada Kanchan and Jayachandra , 1981. Allelopathic effects of Parthenium hysterophorus L. Part V. Mode of action of inhibitors in the substratum . Can.J. Bot 59: 199-202. Important publication in pipeline: 1. Sukhada Mohandas, H.D.Sowmya, A.K.Saxena, Tilaka Rani and Meenakshi S. 2013. Transgenic banana cv Rasbale (syn Rasthali AAB Silk gp) harboring AMP gene from onion seeds imparts enhanced resistance to Fusarium oxysporum f.sp. cubense Race 1 in pot culture. 2.

Important Publications: 
1. Sukhada Kanchan and Jayachandra, 1979. Allelopathic effects of Parthenium hysterophorus L. Part III . Effect of weed residue on crop growth. Plant and Soil, 53, 37-47. 2. Sukhada Kanchan and Jayachandra , 1980. Allelopathic effects of Parthenium hysterophorus L. Part IV. Identification of inhibitors. Plant and Soil. 55. 67-75. 3. Sukhada Kanchan and Jayachandra , 1981. Allelopathic effects of Parthenium hysterophorus L. Part V. Mode of action of inhibitors in the substratum . Can.J. Bot 59: 199-202. 4. Sukhada Kanchan and Jayachanadra, 1980. Pollen Allelopathy- A new phenomenon. New Phytologist 84(4), 739-746 5. Sukhada Mohandas, 1985. Effect of presowing seed treatment with molybdenum and cobalt on growth, nitrogen and yield in bean (Phaseolus vulgaris L.). Plant and Soil, 86, 283-285. 6. Sukhada Mohandas, 1987. Field response of tomato (Lycopersicum esculentum Mill. &lsquo;Pusa Ruby&rsquo; to inoculation with VA myjcorrhizal fungus Glomus fasciculatum and with Azotobacter vinelandii. 1987. Plant and Soil. 295-297</p> 7. Sukhada Mohandas 1988. Nitrogen fixation in tomato (Lycopersicon esculentum Mill.) Plant and Soil, 107 (2), 219-234
Awards & Recognitions, Patents, Inventions, etc: 

1. AWARDS/ RECOGNISIONS • Recipient of Dr.Gopinath Memorial Gold Medal for obtaining Ist rank in M.Sc- 1972 • Recipient of Jawaharlal Nehru Award for the best Ph.D thesis -1980 • Indian National Science Academy’s Young Scientist Award- 1981 • Indian National Science Academy -French Academy of Science’s Exchange fellowship -1987 • Department of Biotechnology’s National Associateship- 1987-88 • C.N.Patel Industrial Award for women scientists by VASVIK foundation-1993. • Punjabrao Deshmuk Outstanding Woman Agricultural Scientist Award 1998 • Fellow of AIPUB – 2008 (Association for the Improvement in Production and Utilisation of Banana) Technologies released 1. Biofertilisers for Horticultural crops. Strains of beneficial bacteria and fungi like fixers, phosphate solubilisers, Arbuscular Mycorrhizal (AM) fungi, identified for fruit crops and popularised and commercialised. 2. On-farm inoculum Production of AM fungi on fingermillet as host standardised, demonstrated in farmers field and Commercialised. 3. Biocontrol package for Fusarium infecting Banana and Phytophtora infecting papaya was developed, field demonstrated. 4. Technique to regenerated banana cv Nanjangud Rasbale from Embryogenic cell suspensions of were standardised. One ml of cell suspension being able to produce 2000 -2500 plants. 5. Genes like antimicrobial peptide , plant ferrodoxine like protein (pflp) and defensine cloned from plant sources, construct developed and used in plant transformation, and also given to other laboratories in the country. 6. Transgenic banana cv Rasthali with AMP gene was developed and short listed for resistance to Fusarium oxysporum pv cubense race 1. With RCGM clearance and NOC from State govt they are being field tested (Event Selection) . 7. Techniques for large scale micropropagation of Elakki Bale and Rasbale developed with higher regeneration efficiency

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Contact Details
Address for Communication: 
15, Anugraha, II cross, Atmananda Colony, Sultanpalya, Bangalore 560 089. Telephone #: 080 - 284466420 ext. 403 (R) 080-23336774
Email Address: 
sukhada@iihr.ernet.in
Telephone #: 
28466420

Aswath, C.

Designation: 
Principal scientist(Horticulture)
Division/Section: 
Division of Biotechnology
Specialisation: 
Horticulural Biotechnology
Qualification: 
Ph.D
Experience (No. of Years): 
22
Permanent Address: 
29/44, 5th cross, wilson Garden, Bangalore 560027
Research Accomplishments: 

Varieties released: 8 varieties were released in Flower crops. Chrysanthemum - Arka Swarna and Usha Kiran for novelty and higher yield Gerbera : Arka krishika, novel variety which can perform on par with exotic varieties outside the polyhouse Crossandra - Arka Ambara, Arka Shreeya,Arka Shravya and Arka Kanaka. First three have been commercialized and large quantity of saplings has been delivered Gladiolus;Arka Amar, a novel variety New Concept • MADS box gene which helped to define conditions that permit plant morphogenesis to occur in vitro has been demonstrated using transgenics(Chenna Reddy Aswath , Sug Youn Mo, Sun-Hyung Kim, Doo Hwan Kim (2004) IbMADS4 regulates the vegetative shoot development in transgenic chrysanthemum (Dendrathema grandiflora (Ramat.) Kitamura) Plant Science, 2004 166: 847–854.) Processes • Identification of Citrus rootstocks using SCAR markers- Govt of Maharashtra is commercializing this technology by cerifying each grafted plant to be tested for proper use of root stock. Filing of patent is in process • Quick detection of Phytoplasma in gerbera using PCR- it will be helpful in detecting the samples especially in quarantine • Transformation in Onion without antibiotics: This is used for transformation and it is quoted 40 times in leading journals • Hybrid purity test for Arka Meghana an F1 Hybrid Chilli: National seed corporation used our technology and sold 6 Q of F1 hybrid seed based on the certification of Hybrid purity • Barcodes developed in guava, Brinjal and Onion. Most of the private seed companies are getting DNA finger printing in our lab for registration of varieties Tissue culture: Tissue culture has been standardized in Anthurium, Gerbera, Orchids and chrysanthemum. Number of papers has been published in these protocols. The technology has been transferred to many small enterpruners. The technology has been displayed in a business meet (24th Nov 2012) and number of small enterpruners have shown the interests to purchase the technology.

Important Publications: 

1 Chenna Reddy Aswath , Sug Youn Mo, Sun-Hyung Kim, Doo Hwan Kim (2004) IbMADS4 regulates the vegetative shoot development in transgenic chrysanthemum (Dendrathema grandiflora (Ramat.) Kitamura) Plant Science,166: 847–854. 2 Chenna Reddy Aswath, Doo Hwan Kim, Sug Youn Mo, Se Won Park (2006) Agrobacterium and biolistic transformation of onion using non-antibiotic selection marker phosphomannose isomerase Plant Cell Reports, 25(2):92-99 3 Hemavathi, Chandrama, Young, Akula, KimHeung, Man Oh, Aswath, Chun, Kim, Park (2009)Over expression of strawberry D-galactronic acid reductase in Potato leads to accumulation of Vitamin C with enhanced abiotic stress tolerance . Plant Science,177(659-667) 4 Chenna Reddy Aswath, Sun Hyung Kim, Sug Youn Mo, Doo Hwan Kim(2005) Transgenics of creeping bent grass harboring stress inducible gene 9-cis-epoxycarotenoid dioxygenase are highly tolerant to water and salt stress. Plant Growth Regulation, 47:129–139 5 Chenna Reddy Aswath and Sun Hyung Kim (2005).Another story of MADS-box genes - their potential in plant biotechnology. Plant Growth Regulations, 46:177–188 6 Park.S.W, J.H. Jeon, H. S. Kim, Y.M. Park, C.Aswath and H. Joung.( 2003)Effect of sealed and vented gaseous microenvironments on the hyperhydricity of potato shoots in vitro Scientia Horticulturae, 99: 197-203 7 Nhut D.T., D.A. Silva Jat, C.R.Aswath (2003) The importance of explant on regeneration in thin cell layer technology In vitro cellular and development biology Plant, 39:3, 266-276 8 Dhandapani, M, S.B. Hong, C.Aswath and D.H.Kim (2007). Regeneration of Zoysia grass(Zoysia matrella L.Merr.)cv.Konhee from young inflorescence and stem nodes. In vitro cell Dev. Biol. Plant.90(21-27) 9 Duong T.Nhut, Bui V.Le, Jaime, A. Teixeira Da Siva and C.Aswath (2001)Thin cell layer culture system in Lilium: Regeneration and Transformation perspectives. In vitro Cell Developmental Bilogy- Plant, 37:516-523 10 Park,S.W., Jeon,J.H., Kim, H.S., Hong, S.J, C. Aswath and Hyouk, J.(2009) The effect of size and quality of potato microtubers on quality of seed potatoes in the cultivar ‘Superior’, Scientia Horticulture 120(1):127-129 11 Aswath, C and M.L.Choudhary. 2002. Plant regeneration from callus cultures of G.jamesoni . Acta Botanica Croat. 61(2) 125-134.

Awards & Recognitions, Patents, Inventions, etc: 

• Best poster award for the research paper on molecular characterization of Crossandra varieties/hybrids/species using ISSR markers in Swadesh Prem Jagriti Sangosthi held at UHS, Bangalore from May 28-31, 2010. • Development of molecular marker for pulp colour in guava” by was adjudged as the best poster (Session-II) during the Horticultural Congress held at New Delhi from 18-21 Nov. 2010. • Best Oral Presentation award for Breeding Anthurium for scent and disease resistance. National symposium on Recent advances in Floriculture held during March 4-6th 2007 in Navasari, Gujarat • Awarded Post Doc fellowship on Transformation of Ornamental crops in Konkuk University , Seoul South Korea under KOSEF from 17th June 200l to 16 June, 2003(2years). • INSA-JSPS exchange fellowship in AICHI University in Japan for period of three months (1998-99) • Senior Research Fellowship for PG research(1983-85) • Merit Scholarship for undergraduate studies(1979-83)

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Contact Details
Email Address: 
aswath@iihr.ernet.in
Telephone #: 
28466420

Anuradha Sane

Designation: 
Principal Scientist
Division/Section: 
Division of Plant Genetic Resources
Specialisation: 
Breeding, Molecular characterization
Qualification: 
Ph.D (Horticulture)
Experience (No. of Years): 
17 Yrs
Research Accomplishments: 

Evaluated and identificated of Bird of Paradise accessions with specific attributes, which can be used as clonal selections.
Generated mutant population in bird of paradise by chemical mutagenesis mutants and gamma irradiation for isolating mutants with desirable traits.
Molecular characterization and genetic diversity of Ornamental crops (Rose, Carnation, Crossandra, Tuberose and Gladiolus) germplasm using SSR/ISSR markers.
Optimized DNA isolation and PCR protocol in Rare Endangered Threatened (RET) medicinal plant species.
Molecular characterization and genetic diversity carried out in some RET plant species collected from different geographical locations.
Molecular characterization and genetic diversity analysis in Jack fruit (Artocarpus heterophyllus).
For Genetic Fidelity testing of tissue culture plants identified polymorphic primers in Carnation, anthurium, Cala lily and gerbera using molecular markers.
Distinguishing zygotic seedling in polyembryonic (PE) varieties of mango using SSR markers.

Important Publications: 

Anuradha Sane, Anuradha Upadhyay, R Manimekalai and VA Parthasarathy, (2003). An
efficient method of DNA extraction from Theobroma cacao l. and cola nitida leaves. Agrotropica, 15(2): 127-130
Anuradha Sane, A. Chandra Sekar and R. Manimekalai, 2005. Optimal PCR parameters for
RAPD analysis in Theobroma cacao and Cola nitida. Plant Cell Biotechnology and Molecular biology 7(1&2): 81-84.
Anuradha Sane and A. Chandra Sekar, 2006. Molecular Characterization of Cocoa (Theobroma
cacao L) germplasm using RAPD markers. Plant Cell Biotechnology and Molecular biology 7(3&4): 151-54.
Anuradha Sane and T.Janakiram Sensitivity and mutagenic effects of different chemicals on the
growth of Bird of paradise (Strelitzia reginae) 2010. Journal of Ornamental Horticulture 13 (4) 247-250
Anuradha Sane, V.K. Abdul Kareem, P.E.Rajasekharan and T.Vasant Kumar. Optimization of
DNA Isolation and PCR Protocol for ISSR Analysis of Nothapodytes nimmoniana, a Threatened Anti-cancerous Medicinal Plant. Asian journal of biotechnology 4 (2); 100-107.
Tejaswini, Madhavilatha, P., Anuradha, S., Dhananjaya , M.V. and Sudhagar, S. 2012.
Microsatellite markers in comparison with morphological characters for protection of plant cultivars in carnation. Acta Horticulture (ISHS) 929:453-458

Important Publications: 
Anuradha Sane, Anuradha Upadhyay, R Manimekalai and VA Parthasarathy, (2003). An
Awards & Recognitions, Patents, Inventions, etc: 

Awarded Gold Medal for securing highest marks in the M.Sc. programme in Horticulture by University of Agricultural Sciences, Bangalore.

Awarded with ICAR Young Scientist award in Horticulture for the biennium 1997-98

Awarded best poster paper in National conference on horticultural biodiversity for livelihood, economic development and health care, 28-31 May 2010, Bangalore, Karnataka.

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Contact Details
Address for Communication: 
PGR division, IIHR, Bengaluru 560089
Email Address: 
anuradha@iihr.ernet.in
Telephone #: 
28466420 extn382

Bhanuprakash, K. Dr

Designation: 
Principal Scientist (pl.physiology)
Division/Section: 
Section of Seed Science & Technology
Specialisation: 
Seed Biochemistry & Molecular Biology, Seed purity, seedquality improvement and proteomics; project work training
Qualification: 
Ph.D (Plant Physiology) moecularlbiology & biochemistry (minors)
Experience (No. of Years): 
16
Research Accomplishments: 

Crop production relies heavily on quality seeds and hence it is to be ensured that each and every seed that reaches to the field for sowing is filled with quality attributes. Thus testing, improvement and maintenance for quality assumes paramount importance in seed science. Our current programmes deals with these aspects at physiological, biochemical and molecular level to evaluate and enhance quality of seed lot. In this connection various protocols (biochemical and molecular) have been developed for genetic purity in chilli, onion, okra and other crops. The reasons for loss in viability in various crops(onion capsicum ,beans ,carrot etc ) were identified majorly on changes membrane permeability, lipid peroxidation, protein expression, enzyme stability, DNA integrity etc etc and rectifying the same by adopting simple and most economical seed priming technology. The advancement in germination and enhancement in vigour through seed priming in low vigour seeds upon priming was investigated at sub cellular level in various crops and crop/ specific protocols were developed. Besides these, seed germination problems in rose, guva and khirni were addressed. Developed seed standards in various medicinal crops and protocols for germination enhancement in various medicinal crops were developed.

Important Publications: 

Bhanuprakash, K., Tejeswini, H.S. Yogeesha & L.B.Naik Effect of scarification and gibberellic acid on breaking dormancy in rose seeds. Seed Research, 2004. 32(1): 5-8. Bhanuprakash, K., C. Vasugi, H.S. Yogeesha, M.N. Arun and L.B. Naik Studies on seed germination and other seed related traits in various Guava accessions. Andhra Agricultural Journal, 2005. 52(1): 61-64 . Bhanuprakash, K. H.S. Yogeesha, L.B. Naik and M.N. Arun. Studies on physiological and biochemical changes in relation to seed viability in aged onion seeds. Journal of Horticultural Sciences, 2006. 1(1): 15-19 Bhanuprakash, K., Yogeesha, H.S and L.B.Naik Studies on seed quality in relation to ageing and priming in French bean (Phaseolus vulgaris L.) cv. Arka Komal. Fourth International Food legumes Research proceedings . K.Bhanuprakash, H.S.Yogeesha, C.Vasugi, M.N.Arun and L.B.Naik 2008 Effect of pre-soaking treatments and temperature on seed germination of guava (Psidium guajava L.) Seed science and technology 36(3):792-94 Bhanuprakash, K., Yogeesha, H.S., Arun, M.N. and Naik, L.B. 2009. Effect of priming and ageing on seed germination and vigour in papaya (Carica papaya L). Indian journal of Agricultural Sciences: 79(4).295-297. Bhanuprakash, K.,Yogeesha, H.S and Arun, M.N (2010). Physiological and biochemical changes in relation to seed quality in ageing bell pepper (Capsicum annum L.) seeds Indian Journal of Agricultural Sciences.80(80) 10-13 K.Bhanuprakash, H.S.Yogeesha, M.N.Arun and L.B.Naik.2008. Effect of storage and priming on seed viability and vigour in Khirni (Manilkara hexandra L) . Seed research 36: (1): 47-50

Patents, Inventions, Technology Developed: 
technologies: 1. seed germination enhancement in rose,guava and khirni and medicinal crops 2. seed viability enhancement of low vigour seeds of onion,carrot,benas,bottle gourd,capsicum etc through seed priming 3. protocols for genetic purity testing in chilli,onion
Awards & Recognitions, Patents, Inventions, etc: 

Received best presentation awards(12) Recognized as PG teacher in various universities

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Contact Details
Address for Communication: 
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